Pyrenomycetes Species Page

Lasiosphaeria lanuginosa

Large clustered ascomata
didymospore
(H. Crouan & P. Crouan) A.N. Mill. & Huhndorf, Mycol. Res. 108(1): 31. (2004)
Sordaria lanuginosa P. Crouan & H. Crouan, Florule Finistère (Paris): 22 (1867)
Sordariomycetidae
Sordariales, Lasiosphaeriaceae
Figure from SMH3277, SMH3819 & SMH4027 Type Specimen: FRANCE. on Cirsium palustre stems, 5/17/1864, (HOLOTYPE, CO) (as Sordaria lanuginosa P. Crouan & H. Crouan) Material Examined: multiple collections
Genbank Accession: AY587917 (ITS), AY587940 (LSU), AY600258 (Btub), AY600280 (RPB2)
Complete Description
Ascomata ampulliform to ovoid, papillate, 360–640 µm diam, 360–740 µm high, numerous, scattered to gregarious, usually superficial, rarely erumpent; with cottony tomentum, hairs occasionally spreading at base, occasionally whitish to commonly pale yellowish or occasionally pale pinkish, sometimes becoming orange-brown with age, occasionally wearing away so that only a few yellowish hairs remain; neck conical, glabrous, black. – Ascomatal wall of textura angularis in surface view, commonly containing globular, refractive crystals in squash mounts, in longitudinal section 3-layered, 48.5–71.5 µm thick, inner layer pseudoparenchymatous, 7.5–9 µm thick, composed of 3–5 layers of elongate to flattened, hyaline to brown cells, middle layer pseudoparenchymatous, 20.5–22 µm thick, composed of 4–7 layers of polygonal to angular, brown cells, outer layer prosenchymatous, 20.5–40.5 µm thick, composed of several to few layers of hyphae, hyphae 1.5–3 µm wide, hyaline to pale brown, septate, thin-walled. – Ascomatal apex with periphyses. – Centrum with yellow to yellowish-orange pigments which quickly diffuse in water. – Paraphyses filiform to ventricose, 2–5 µm wide, longer than asci, hyaline, numerous, septate, unbranched, persistent, contents with blue pigments in SMH4027 only. – Asci cylindrical, 120–230 (–300) X 8–20 (–22.5) µm, medium- to long-stipitate, stipe (26–) 38–91 X 3–7.5 µm, numerous, unitunicate, thin-walled, apex truncate; ring narrow, shallow, refractive; subapical globule large, 3.5–6 µm diam, smooth, with 8, biseriate to tetraseriate ascospores. – Ascospores cylindrical, ends rounded, 33–60 X 2.5–5 µm [44.5 ± 6.5 X 4 ± 0.5], slightly sigmoid or geniculate, lower 1/3 bent 45° or less, hyaline, aseptate; with bipolar appendages, 7–30 (–41.5) µm, gelatinous, lash-like; becoming differentiated into a swollen head and pedicel while inside the ascus, transversely uniseptate; head ellipsoid to ovoid, 11.5–19 X 6–10.5 µm, subacute to rounded at the apex, truncate at the base, hyaline to occasionally brown; pedicel 23.5–32.5 X 3–5 µm, hyaline to rarely pale brown, 1-septate; head brown and rarely 1-septate after liberation from the ascus, ascospore 1- to 2-septate after liberation from the ascus. (from Miller & Huhndorf, 2004)
Occurrence
Found on the decorticated parts of dead, deciduous branches which still possess remnants of adhering bark, or rarely on dead leaves or grass throughout the world including Costa Rica, Denmark, England, France, Peru, USA, and Venezuela.
Similar Taxa Comments
Lasiosphaeria lanuginosa is a species complex that can usually be distinguished by having ascomata covered with a dense, white to yellowish or rarely pale pinkish tomentum and ascospores which usually develop a swollen head and possess lash-like appendages. It can be distinguished from Lasiosphaeria ovina by the development of a swollen ascospore head that sometimes turns brown and its longer ascospore appendages. Cercophora sparsa can be separated by its shorter ascospores (29.5–37 vs. 33–60) which lack appendages. This species forms a polyphyletic species complex in which most specimens possess a combination of ambiguous characters making it difficult to identify collections. See Miller & Huhndorf (2004) for more details.
Reference
Miller, A.N. and S.M. Huhndorf. 2004. Using phylogenetic species recognition to delimit species boundaries and species relationships within Lasiosphaeria. Mycologia 96(5): 1106–1127.